Journal Entry: Term 3, Week 3 -- Day 1
Ok, I haven't been posting on this blog since the end of last term so I've decided to blog once now.
Ok basically the past two weeks i won't blog too much here but just ot let you know that we didn't really do anything worthy of special mention here. I'll post again on the past two weeks seperately.
Terms to note before I begin writing:
1. PBS - Phosphate Buffer Saline (Its isotonic to the zebra fish embro)
2. MEOH is Methanol but in most cases i'll use methanol instead of MEOH
3. PBDT: Long name, P - Phosphate buffer saline, B - Bovine Serum Albumen, D- DMSO, T - Triton X100
4. For the benefit of any1 hu wants to noe, DMSO stands for DiMethyl SulfOxide
Today was the first day of the IMCB lab attatchment. It was sorta boring the first day, thanks to the training ACS(I) people get in sec 1 and 2 (Not pointing fingers either). They started out with 2hrs of briefings which are not too boring, but extensive. Oh yes, before i forget, the title of the course goes "Diversificaton of Muscles and Neurons in Animal Development". Then they moved on to a MICROPIPPETTE EXCERCISE!!! Yes!!! Something we've been doing for a long time in sec 1 and 2, we did again during the excercise with distilled water!!! Well, after that was like 2hrs of lunch time ( not that we were really hungry).
After coming back from lunch the real stuff begins, where the zebra fish embryos, which we're supposed to find the muscle proteins in them, are to be experimented on (evil laugh... just joking). First is to remove the methanol they're being kept in and replace with 50% methanol:PBS mix. Then sit for 5mins, then drain and fill with 1ml of PBDT solution. Then sit for 2 mins, then remove solution and then refill with 1 ml of PBDT again and repeat this 3 times for a total of 4 washes. Then fill with 250 microlitres of blocking solution (Apparently PBDT again) and place on the shaker for 1hr. 1HR!!!! we started to slack and waste time during that 1hr (which is why i am bringing my comp tomorrow). After which, just to remove the solution and add primary antibody solution and store at 4 degrees celsius, after which we could go home. Slack and really boring, though tomorrow should be much more interesting. Will blog again tomorrow.
Btw, we discovered today that scientists aren't always clear headed people.... er.. find out from me in class...
For all budding biologists, here's a question for you to ponder.. what's the difference between a mutagen and a teratogen? Hint: its in the which stage of coding they affect. I'll give you the answer tomorrow... Tired now.. bye!
Ok basically the past two weeks i won't blog too much here but just ot let you know that we didn't really do anything worthy of special mention here. I'll post again on the past two weeks seperately.
Terms to note before I begin writing:
1. PBS - Phosphate Buffer Saline (Its isotonic to the zebra fish embro)
2. MEOH is Methanol but in most cases i'll use methanol instead of MEOH
3. PBDT: Long name, P - Phosphate buffer saline, B - Bovine Serum Albumen, D- DMSO, T - Triton X100
4. For the benefit of any1 hu wants to noe, DMSO stands for DiMethyl SulfOxide
Today was the first day of the IMCB lab attatchment. It was sorta boring the first day, thanks to the training ACS(I) people get in sec 1 and 2 (Not pointing fingers either). They started out with 2hrs of briefings which are not too boring, but extensive. Oh yes, before i forget, the title of the course goes "Diversificaton of Muscles and Neurons in Animal Development". Then they moved on to a MICROPIPPETTE EXCERCISE!!! Yes!!! Something we've been doing for a long time in sec 1 and 2, we did again during the excercise with distilled water!!! Well, after that was like 2hrs of lunch time ( not that we were really hungry).
After coming back from lunch the real stuff begins, where the zebra fish embryos, which we're supposed to find the muscle proteins in them, are to be experimented on (evil laugh... just joking). First is to remove the methanol they're being kept in and replace with 50% methanol:PBS mix. Then sit for 5mins, then drain and fill with 1ml of PBDT solution. Then sit for 2 mins, then remove solution and then refill with 1 ml of PBDT again and repeat this 3 times for a total of 4 washes. Then fill with 250 microlitres of blocking solution (Apparently PBDT again) and place on the shaker for 1hr. 1HR!!!! we started to slack and waste time during that 1hr (which is why i am bringing my comp tomorrow). After which, just to remove the solution and add primary antibody solution and store at 4 degrees celsius, after which we could go home. Slack and really boring, though tomorrow should be much more interesting. Will blog again tomorrow.
Btw, we discovered today that scientists aren't always clear headed people.... er.. find out from me in class...
For all budding biologists, here's a question for you to ponder.. what's the difference between a mutagen and a teratogen? Hint: its in the which stage of coding they affect. I'll give you the answer tomorrow... Tired now.. bye!
